&lt;i&gt;In vitro&lt;/i&gt; shoot proliferation and development of micropropagation protocol from leaf disc of Gladiolus

M Shaheenuzzaman,Mm Karim,Ms Haque,Zu Noor

doi:10.3329/jbau.v9i1.8739

Abstract

The experiment was carried out during the period from July 2008 to March, 2009 at the Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh. The present investigation was to be better source for shoot multiplication. The calli derived from leaf discs were cultured on shoot induction media containing different combinations and concentrations of BAP (1.0, 1.5, 3.0 and 6.0 mg/L) and Kn (0.5, 1.0, 1.5 and 2.0 mg/L). The highest percentage of shoot regeneration (91.66%) and the highest shoot length was 4.24 (cm) with a minimum number of days (13.33) was observed in the MS medium supplemented with 3.0 mg/L BAP and 0.5 mg/L Kn. Thus shoot multiplication in successive subculture was possible. The present investigation was carried out to study the in vitro shoot multiplication in gladiolus by using leaf disc as explants Keyword: Shoot proliferation; Micropropagation; BAP: 6-benzylamino purine; Kn: Kinetin; Leaf disc DOI: http://dx.doi.org/10.3329/jbau.v9i1.8739 JBAU 2011; 9(1): 21-26

Highlights

Gladiolus perennates by underground stems or corms, the infection by soil-borne fungus, Fusarium oxysporum f. sp. gladioli causing severe loss and damage is a major bottleneck to its mass propagation
The propagation by corm and cormel formation is another conventional method of multiplication but it may transmit several viral, fungal and bacterial diseases e.g., Fusarium corm rot, Botrytis blight, bacterial leaf rot etc., causing a heavy loss
Studies were conducted with gladiolus in order to standardize the protocol for shoot proliferation in vitro

Summary

Introduction

Gladiolus perennates by underground stems or corms, the infection by soil-borne fungus, Fusarium oxysporum f. sp. gladioli causing severe loss and damage is a major bottleneck to its mass propagation. With the growing symbiosis between plant breeders and biotechnologists, disease resistant varieties of Gladiolus developed through plant transformation will be a reality in near future and some reports are already available (Kamo, 1997; Kamo and Blowers, 1999; Loffler et al, 2000). It propagates either by seeds, corm formation or by Cormel differentiation. Seeds are an effective means of Gladiolus propagation but seed-raised plants may not produce true-to-type population (Hussain et al, 2001). The propagation by corm and cormel formation is another conventional method of multiplication but it may transmit several viral, fungal and bacterial diseases e.g., Fusarium corm rot, Botrytis blight, bacterial leaf rot etc., causing a heavy loss

Methods

Results

Conclusion