Abstract
The experiment was carried out during the period from June 2016 to January, 2017 at the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University (PSTU) for in vitro regeneration of sweet potato. In this study, the nodal segment explants and different combinations of growth regulators were used for in vitro regeneration of sweet potato. BAP (6-benzylamino purine) and KIN (Kinetin) were used for in vitro shoot regeneration and IBA (Indole-6- Butyric Acid) with NAA (Naphthalene Acetic Acid) were used for in vitro root regeneration. The highest percentage of shoot initiation (91.30), the minimum number of days (9.00) for shoot initiation, the highest number of shoot/plantlet (11.00) and the highest shoot length (4.38 cm) was observed when nodal segment explants cultured on MS medium supplemented with BAP 1.5 mg/L + KIN 0.1 mg/L. On the other hand, the highest root initiation percentage (94.12), the minimum number of days (6.00) for root initiation, the highest number of root/plantlet (9.33) and the highest root length (11.13 cm) was observed in the MS medium supplemented with IBA 0.5 mg/L + NAA 0.1 mg/L. Regenerated plantlets were acclimatized for 5 days and adopted in soil by 6-7 days of transplantation.J. Bangladesh Agril. Univ. 16(2): 178-181, August 2018
Highlights
The sweet potato (Ipomoea batatas L.) is herbaceous dicotyledonous plant that belongs to the family Convolvulaceae
Days required for Number of shoot Shoot length percentage shoot initiation per explant
Values having different letter (s) differ significantly at 5% level of probability analyzed by DMRT, BAP= 6-benzylamino purine, KIN= Kinetin
Summary
The sweet potato (Ipomoea batatas L.) is herbaceous dicotyledonous plant that belongs to the family Convolvulaceae. Aphids and whitefly vectors are the main causes of virus transmission (Moyer and Salazar, 1989; Gibson, 2004) This conventional system is characterized by low multiplication rate, wastage of a large quantity of food material, absence of uniformity, risk of catching diseases. Investigations on the utilization of tissue culture technology for exchange and conservation of germplasm of sweet potato were conducted (Jemal, 2009).The success in production of the healthy or disease free planting material of sweet potato is dependent on the reliability and efficiency of the regeneration protocol used. The present study was undertaken to develop a suitable in vitro regeneration protocol and to determine the optimal concentrations and combinations of plant growth regulators for shoot and root initiation of sweet potato from nodal segment explants
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