Abstract
LINC00599 has been suggested to be involved in physiological and pathological processes including carcinogenesis. However, the clinical and prognostic significance of LINC00599 in glioma patients and the effect of LINC00599 on glioma cell migration and invasion remain unknown. In our results, we first observe the expression of LINC00599 in 31 types of human cancers including tumor tissues and corresponding normal tissues at The Cancer Genome Atlas (TCGA) database, and found that LINC00599 expression levels were only reduced in lower grade glioma (LGG) tissues and glioblastoma multiforme (GBM) tissues compared with normal brain tissues. Moreover, we confirmed levels of LINC00599 expression were decreased in glioma tissues and cell lines compared with matched adjacent normal tissues and normal human astrocytes (NHAs), respectively. Meanwhile, we found that glioma tissues with WHO III-IV grade exhibited lower levels of LINC00599 expression than glioma tissues with I-II grade. The survival analysis at TCGA data showed low LINC00599 expression was associated with poor disease-free survival and overall survival in glioma patients. In vitro study suggested up-regulation of LINC00599 depressed glioma cell migration and invasion through regulating epithelial–mesenchymal transition (EMT) process. In conclusion, LINC00599 acts as a tumor-suppressing long non-coding RNA (lncRNA) in glioma.
Highlights
Glioma is the most common malignancy of the central nervous system accounting for approximately 296851 newly diagnosed cases worldwide in 2018 [2]
LINC00599 expression is reduced in glioma tissues and cell lines
For estimating the clinical significance of LINC00599 expression in glioma patients, we grouped 60 glioma tissues based on age (
Summary
Glioma is the most common malignancy of the central nervous system accounting for approximately 296851 newly diagnosed cases worldwide in 2018 [2]. Glioma accounts for 2.5% of all cancer deaths (approximately 241037) worldwide in 2018 [2]. More and more lncRNAs have been suggested to be dysregulated in glioma tissues and involved in regulating glioma cell proliferation, differentiation, apoptosis, cell cycle, and autophagy [21,22,23,27]. LINC00599 ( known as retinal non-coding RNA3) is an lncRNA transcribed from the intergenic regions of the genome and is conserved in mammals [5,25]. LINC00599 was found to be dynamically expressed and acts as a potential regulator of neurons and oligodendrocyte differentiation during retinal development [12].
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