Invasion and migration regulation of human glioma cells by long non-coding RNA linc00996

Abstract

Objective To investigate the expression of long non-coding RNA linc00996 in glioma, its function in migration, invasion and its relationship with prognosis. Methods The differential expression of linc00996 in glioma patients and normal brain tissues was compared in TCGA database. 21 patients with glioma treated in our hospital were recruited. Tumor tissues and normal brain tissues were collected in the operation. The expression level of linc00996 in the above tissues was detected by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR). Small interfering RNA targeting linc00996 sequence was synthesized and transfected into human glioma cell line U251. Wound healing and Transwell assay were used to detect the effect of down-regulation of linc00996 on migration and invasion of U251 cells respectively. In TCGA database, according to the median expression of linc00996 in glioma tissues, the patients were divided into two groups to compare the overall survival and disease-free survival of the linc00996 high and low expression groups. Statistical Analysis with STATA 12.0 Software. Results TCGA database showed that the expression level of linc00996 in glioma tissues was higher than that in normal brain tissues. In 21 cases of glioma, the expression level of linc00996 in tumor tissues was higher than that in normal tissues in 15 cases (71.4%). Small interfering RNA (si00996) could significantly down-regulate the expression of linc00996 in U251 cells (t=8.870, P<0.05). Wound healing assay showed that the cell migration ability decreased significantly after down-regulation of linc00996 expression. Transwell assay showed that the ability of U251 cells to penetrate membranes decreased after small interfering RNA silenced linc00996. Conclusion Linc00996 is highly expressed in glioma tissues and is associated with poor prognosis. Down-regulation of linc00996 expression can inhibit the migration and invasion of glioma cells. Key words: Glioma; Long non-coding RNA; Migration; Prognosis