Expression of microRNA-542-3p in human glioma and its effect on cell proliferation and migration

Abstract

Objective To evaluate microRNA (miRNA, miR)-542-3p expression in glioma and its effect on cell proliferation and migration. Methods The expression of miR-542-3p in tumor tissues of 33 patients with glioma and 29 normal brain tissues of patients with traumatic brain injury was examined by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detect. Synthesize miR-542-3p mimics and unrelated sequences (miR-542-3p NC) were transfect into human glioma cells (U87) by Lipofectamine 2000, respectively. Cell proliferation, migration and invasion ability was examined by cell counting kit-8 (CCK-8), wound healing and Transwell assay respectively. Results The relative expression levels of miR-542-3p in glioma and normal brain tissues were 0.74±0.06 and 1.06±0.09, respectively. The relative expression levels of miR-542-3p in glioma tissues were significantly lower than those in normal brain tissues (t=3.03, P 0.05wever, after transfection of miR-542-3p mimics into U87 cells, the migration and invasion ability of cells increased significantly. Conclusion MiR-542-3p expression in human glioma cells is decreased, and inhibite cell migration and invasion in glioma cells. Key words: Glioma; MicroRNA-542-3p; Proliferation; Invasion; Metastasis