Expression of cell cycle-related protein in glioma tissue and it effect on cell viability

Abstract

Objective To investigate the expression of cell cycle-related protein (CAPRIN1) in glioma and the effect on cell activity. Methods Thirty-four specimens of brain glioma tissue resected in our hospital from June, 2016 to December, 2017 were selected. Immunohistochemical method was used to detect the expression of CAPRIN1 in 3 normal brain tissue specimens from autopsy at the same time. The of CAPRIN1 expression was detected by immunohistochemistry. Logarithmic human glioma cell line GOS-3 was divided into three groups: a blank control group (conventional culture without any treatment), a negative control group (transfection of pEGFP-N1 empty vector plasmid), and an observation group (transfection of pEGFP-N1-CAPRIN1 plasmid). CCK-8 method and Transwell chamber were used to detect cell proliferation and migration ability; and Western blot was used to detect the expressions of AKT, p-AKT, and CyclinD1 proteins. Results The expression level of CAPRIN1 in the glioma tissue was significantly higher than that in the normal brain tissue (P 0.05); the proliferation level of GOS-3 cells in the observation group was significantly higher than that in the negative control group and that in the blank control group at 48 h, 72 h and 96 h (P 0.05). The relative expression of p-AKT protein in the observation group was significantly higher than that in the blank control group and that the negative control group (P 0.05). Conclusion The expression of CAPRIN1 in glioma tissue increases with malignancy. Up-regulation of CAPRIN1 expression can significantly enhance the proliferation and migration of glioma cells; it may be related to the activation of AKT signaling pathway and Cyclin D1 protein. Key words: Glioma; GOS-3 cells; CAPRIN1; Proliferation; Migration