Long non-coding RNA SNHG8 enhances triple-negative breast cancer cell proliferation and migration by regulating the miR-335-5p/PYGO2 axis

Jintao Qian,Jianing Shi,Wenjun Jia,Shuai Zhang,Xinhan Lei,Jia Li,Lu Zheng,Wanwan Li,Tong Tang,Lei Zhang,Yue Sun

doi:10.1186/s13062-021-00295-6

Abstract

BackgroundGrowing evidence has demonstrated that long non-coding RNAs (lncRNAs) can function as modulators in the development of triple-negative breast cancer (TNBC). However, the function of lncRNA small nucleolar RNA host gene 8 (SNHG8) in TNBC remains unclear. Therefore, our study aimed at investigating the role of SNHG8 in the proliferation and migration of TNBC cells.MethodsSNHG8 expression was evaluated using RT-qPCR assay. Cell proliferation and migration were assessed by EdU, colony formation and Transwell assays. The levels of proteins related to EMT process were examined by western blot assay. The interaction among SNHG8, miR-335-5p and pygopus family PHD finger 2 (PYGO2) was detected by RIP assay, RNA pull down assay and luciferase reporter assay.ResultsSNHG8 expression was significantly up-regulated in TNBC cells. SNHG8 silencing obviously inhibited TNBC cell proliferation, migration and EMT process. Moreover, SNHG8 acted as a sponge to sequester miR-335-5p in TNBC cells. Besides, PYGO2 was proven as a target gene of miR-335-5p, and SNHG8 promoted TNBC cell proliferation, migration and EMT process through regulating miR-335-5p and PYGO2.ConclusionsTotally, our study indicated that SNHG8 promoted TNBC cell proliferation and migration by regulating the miR-335-5p/PYGO2 axis.

Highlights

Growing evidence has demonstrated that long non-coding RNAs can function as modulators in the development of triple-negative breast cancer (TNBC)
small nucleolar RNA host gene 8 (SNHG8) facilitates the proliferation, migration and epithelial-mesenchymal transition (EMT) process of TNBC cells To basically understand the function of SNHG8 on the biological behaviors of TNBC cells, its expression in different cell lines was detected
According to the results of Real-time quantitative polymerase chain reaction (RT-qPCR), it was suggested that only Pygopus family PHD finger 2 (PYGO2) was downregulated in TNBC cells transfected with miR-335-5p mimics, we selected PYGO2 for further experiments (Fig. 3A)

Summary

Introduction

Growing evidence has demonstrated that long non-coding RNAs (lncRNAs) can function as modulators in the development of triple-negative breast cancer (TNBC). The function of lncRNA small nucleolar RNA host gene 8 (SNHG8) in TNBC remains unclear. Increasing evidences have demonstrated that the abnormal expression of lncRNAs can function as oncogenes or tumor suppressors to participate in the occurrence and development of cancers, including TNBC. LncRNA small nucleolar RNA host gene 8 (SNHG8) has been confirmed to exert carcinogenic function in many types of tumors. SNHG8 accelerates cell growth through sponging miR-663 in colorectal cancer cells [13]. SNHG8 acts as an oncogene of nonsmall-cell lung cancer via miR-542-3p to regulate CCND1/CDK6 [15]. The molecule mechanism and function of SNHG8 in TNBC remained unclear

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