Long Non-Coding RNA RP1130-1 Suppresses Cell Proliferation, Invasion and Migration Mediated by Downregulation of TGF-β

Abstract

Background: Hepatocellular carcinoma has low levels of long non-coding RNA (LncRNA) RP1130. However, the effects of LncRNA RP1130 in hepatocellular carcinoma still unknown. Materials and Methods: Expression of LncRNA RP1130-1 in HCC and cell lines were detected by real-time PCR. Cell proliferation was assessed by CCK-8. Wound-healing and Transwell assays were performed for HCC cell migration and invasion. Western blotting was carried out to evaluate cell cycle, migration and invasion associated proteins in HCC. Results: Expression levels of LncRNA RP1130-1 was dramatically lower in HCC tissues than in normal control. Similarly, LncRNA RP1130-1 was downregulated in HCC cell lines compared with LO2. The cellular experiments revealed that high expression of LncRNA RP1130-1 in HCC inhibited cell proliferation, migration and invasion. In addition, overexpression of LncRNA RP1130-1 inhibited the expression of transforming growth factor (TGF)-β, and TGF-β reversed the effects of LncRNA RP1130 in HCC cell lines. Conclusions: LncRNA RP1130 exerts anti-tumor effects mediated by inhibiting TGF-β. In summarize, our results indicate that LncRNA RP1130/TGFβ may be a potential therapeutic target for HCC.