MiR-454 functions as an oncogene by inhibiting CHD5 in hepatocellular carcinoma.

Lei Yu,Xuejun Gong,Liying Zhu,Lei Sun,Hong Yao,Baoling Lu

doi:10.18632/oncotarget.4407

Lei Yu, Xuejun Gong + Show 4 more

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https://doi.org/10.18632/oncotarget.4407

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Abstract

Previous studies showed that miR-454 acted as an oncogene or tumor suppressor in cancer. However, its function in HCC remains unknown. In this study, we found that miR-454 expression was upregulated in HCC cell lines and tissues. Knockdown of miR-454 inhibited HCC cell proliferation and invasion and epithelial mesenchymal transition (EMT), whereas overexpression of miR-454 promoted HCC cell proliferation and invasion and EMT. Furthermore, we identified the CHD5 as a direct target of miR-454. CHD5 was downregulated in HCC tissues and cell lines and the expression level of CHD5 was inversely correlated with the expression of miR-454 in HCC tissues. In addition, knockdown of miR-454 inhibited the growth of HepG2-engrafted tumors in vivo. Taken together, these results indicated that miR-454 functioned as an oncogene in HCC.

Highlights

Hepatocellular carcinoma (HCC), the primary cancer of liver, ranks as the fifth most frequent malignancy worldwide, accounting for ~700 000 deaths per year [1,2,3]
We demonstrated that miR-454 expression was upregulated in human cell lines and HCC tissues compared with normal liver cell line and adjacent non-tumor tissue
CHD5 was downregulated in HCC tissues and cell lines and the expression of CHD5 was inversely correlated with the expression of miR-454 in HCC tissues

Summary

Introduction

Hepatocellular carcinoma (HCC), the primary cancer of liver, ranks as the fifth most frequent malignancy worldwide, accounting for ~700 000 deaths per year [1,2,3]. Many studies have identified factors associated with HCC survival; the 5-year survival rate remains quite low among patients with HCC [8,9,10]. MicroRNAs (miRNAs) are endogenous non-coding RNAs (about 22 nt) that have drawn attention in recent years as one of the key modulators [11,12,13,14,15,16]. Recent reports have indicated that deregulation of miRNAs is associated with the formation and progression of many cancers such as gastric cancer, bladder cancer, breast cancer and HCC [30,31,32,33,34]. MiRNAs are potentially useful biomarkers for clinical diagnosis

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