Abstract
BackgroundNon-small cell lung cancer (NSCLC) is one of the most common malignant tumors all over the world. In recent years, long non-coding RNAs (lncRNAs) have been proven to participate in the development of different cancers, including NSCLC. PCGEM1 prostate-specific transcript (PCGEM1) is the lncRNA which is associated with the progression of several cancers. Nevertheless, in NSCLC, the specific functions of PCGEM1 are not yet clear.MethodsThe real-time quantitative polymerase chain reaction (qPCR) was utilized to test the expression of PCGEM1 in NSCLC cells. Functional experiments, including cell Counting Kit-8 (CCK-8) assay, 5-ethynyl-2’-deoxyuridine (EdU) assay, flow cytometry analysis and transwell assays were utilized to estimate cell proliferation, migration, invasion and apoptosis. Meanwhile, RNA pull down assay and luciferase reporter assay were utilized to evaluate the correlation of miR-433-3p with PCGEM1 or WT1 associated protein (WTAP).ResultPCGEM1 was highly expressed in NSCLC cells, while miR-433-3p was lowly expressed in NSCLC cells. PCGEM1 silencing or miR-433-3p overexpression inhibited cell proliferation, migration and invasion but accelerated cell apoptosis. MiR-433-3p was proven be sponged by PCGEM1. Besides, WTAP was the target of miR-433-3p and it accelerated the progression of NSCLC. In the end, rescue experiments indicated that overexpression of WTAP or knockdown of miR-433-3p reversed the inhibited roles of silencing PCGEM1 on cell behavior.ConclusionsPCGEM1 accelerates NSCLC progression via sponging miR-433-3p to upregulate WTAP.
Highlights
Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors all over the world
PCGEM1 prostate-specific transcript (PCGEM1) is highly expressed in NSCLC cells and knockdown of it suppresses cell growth First of all, the expression level of PCGEM1 was examined
With the conduction of EdU assay, we discovered that the ratio of EdU positive cells was decreased in NCIH1299 and NCI-H1650 cells transfected with sh-PCGE M1, which indicated that cell proliferation was restrained by the depletion of PCGEM1 (Fig. 1c)
Summary
Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors all over the world. In recent years, it has become the first cause of cancer-related death in urban population [1]. An increasing number of reports indicate that lncRNAs are frequently to be abnormally expressed in assorted cancers [8]. They exert the functions of tumor suppressor or facilitator in the progression of human
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