Abstract
Simple SummaryZinc finger BED-type containing 6 (ZBED6), as a regulatory factor, has different regulatory mechanisms in animal development. The intron of insulin-like growth factor 2 (IGF2) regulates the development of animal muscle and adipose by combining with the binding site of ZBED6. As a member of the insulin-like growth factor family, IGF2 plays an important role in embryonic growth and development, cell proliferation, muscle growth and genome imprinting. In order to further study the regulatory mechanism of ZBED6 on IGF2, we detected the expression of IGF2 and related genes in ZBED6 single allele knockout (ZBED6-SKO) pig tissues and analyzed differently expressed genes of the transcriptome of ZBED6-SKO pig liver. The results showed that the partial knockout of ZBED6 could affect the secretion of IGF2 in pig liver but had no significant difference at the protein level. This research provides a new idea for the interaction between IGF2 and ZBED6.Zinc finger BED-type containing 6 (ZBED6), a highly conservative transcription factor of placental mammals, has conservative interaction of insulin-like growth factor 2 (IGF2) based on the 16 bp binding sites of ZBED6 on the IGF2 sequence. IGF2 is related to embryo growth and cell proliferation. At the same time, its functions in muscle and adipose in mammals have been widely mentioned in recent studies. To further investigate the mechanism of ZBED6 on IGF2, we detected the expression of IGF2 and related genes in ZBED6 single allele knockout (ZBED6-SKO) pig tissues and analyzed the transcriptome of ZBED6-SKO pig liver. Through RNA-seq, we captured nine up-regulated genes and eight down-regulated genes which related to lipid metabolism. The results showed that the mRNA of IGF2 had an upward trend after the partial knockout of ZBED6 in liver and had no significant difference in protein expression of IGF2. In summary, ZBED6-SKO could affect the secretion of IGF2 in pig liver and its own lipid metabolism. Our research has provided basic information for revealing the regulatory mechanism of the interaction between ZBED6 and IGF2 in mammals.
Highlights
Zinc finger BED-type containing 6 (ZBED6) gene is a transcription regulatory factor newly discovered in recent years [1], which is closely related to the development of muscle and adipose and could regulate the expression of various important function genes
We investigated the effect of ZBED6 knockout on liver Insulin-like growth factor 2 (IGF2) metabolism, expected to provide novel insight for understanding the function of ZBED6 and IGF2 interaction and provide the theoretical basis for lean pig molecular breeding
A mountain of research has shown that the ZBED6-IGF2 axis has an important role in the development of muscle and other tissues [12,14], and the level of IGF2 in the blood was the main focus of attention in their studies
Summary
Zinc finger BED-type containing 6 (ZBED6) gene is a transcription regulatory factor newly discovered in recent years [1], which is closely related to the development of muscle and adipose and could regulate the expression of various important function genes. Insulin-like growth factor 2 (IGF2) is a type of cell-like regulatory factor with insulin-like structure and widely expresses in various tissues during the embryonic stage. The expression of IGF2 is significantly suppressed; it mainly synthesizes in liver and transports to various parts of body. IGF2 has many conservative functions in mammals [3,4,5,6,7]. Its functions are mainly through the combination with insulin-like growth factor 1 receptor (IGF1R) and insulin-like growth factor 2 receptor (IGF2R), or it can compete with insulin-like growth factor binding protein (IGFBP)
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