Abstract
The history of chromatography first began in 1903 and since then, liquid chromatography (LC) has continued to be an important part of many different fields. This article gives an overview of LC instrumentation. The state of art of conventional high-pressure (or performance) LC (HPLC), ultrahigh-pressure LC (UHPLC), micro- and nano-HPLC systems, portable instruments and preparative LC systems is discussed. The main components of the several systems: configurations for sample introduction, pumps, columns and ovens, detectors and hyphenated techniques, are reviewed. LC is an excellent way of producing high quality pure compounds and it is also a dependable way of identifying and quantifying compounds. New LC systems and challenges are also introduced.
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