Abstract
Liquid chromatography—atmospheric pressure chemical-ionization mass spectrometry (LC—APCI-MS) was used for the analysis of N-acetylamino acids that could not be determined with an amino acid analyzer. LC—APCI-MS could directly detect the protonated molecular ions of various synthetic N-acetylamino acids, distinguishing N-acetylserine from O-acetylserine and N(α)-acetyllysine from N(ε)-acetyllysine. Furthermore, N-acetylasparagine, N-acetylaspartic acid, N-acetylglutamine and N-acetylglutamic acid were identified in normal human urine. The assay data for N-acetylaspartic acid agree with a previous report using gas chromatography—mass spectrometry. These results demonstrate the usefulness of the apparatus described above for the analysis of N-acetylamino acids in biological samples.
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