Abstract
Abstract 1. 1. Gravimetric, histochemical, thin-layer- and gas-liquid chromatographic studies were done on lipids and sterols in fresh and starved Corbicula fluminea clams. 2. 2. Gravimetric analysis showed no significant difference in the mean percent lipid of fresh clam bodies (2.2%) versus starved (2.9%). 3. 3. Qualitative thin-layer chromatography (TLC) showed that the main neutral lipid fractions in both fresh and starved clam bodies were free sterols and triacylglycerols along with lesser amounts of sterol esters and free fatty acids. Qualitative differences were not seen in fresh versus starved clams. 4. 4. Quantitative densitometric TLC showed no significant difference in the mean per cent of free sterols in fresh clam bodies (0.09%) versus starved (0.11%). 5. 5. Gas-liquid chromatographic analysis showed cholesterol as the main sterol (61.2%) with lesser amounts of campesterol, β-sitosterol, stigmasterol, and other minor sterols. 6. 6. Oil Red O histochemistry showed the presence of neutral lipids in the visceral mass and gills of both fresh and starved clams.
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