Abstract
1. 1. Histochemical, thin layer and gas-liquid chromatographic studies were done on neutral lipids, sterols and carotenes in the digestive gland-gonad (DGG) complex of Helisoma trivolvis infected with Echinostoma trivolvis vs uninfected DGG. 2. 2. Hitochemical Oil Red O staining showed the presence of neutral lipids in the redial body wall and in the digestive cells of the DGG. 3. 3. TLC showed that free sterols and triacylglycerols were major neutral lipid fractions along with lesser amounts of steryl esters and free fatty acids in the DGG of both populations. The percentage composition of all neutral lipid fractions was greater in infected than uninfected DGG. 4. 4. Infected DGG contained more carotenoid fractions than uninfected DGG, but only beta-carotene was identified from both. 5. 5. GLC studies showed that the major sterol present in snail DGG was cholesterol (about 70%) along with lesser amounts of stigmasterol, campesterol, beta-sitosterol and desmosterol. No clear cut distinction was seen in sterols from infected vs uninfected DGG.
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