Abstract
1. 1. Gravimetric, thin-layer Chromatographic, and histochemical studies were done on lipids in fed and starved Biomphalaria glabrata. 2. 2. Total lipids of fed snails averaged 4.0 mg/snail compared to 3.5 mg in snails starved for 2–3 weeks. The percentage of total lipid basd on snail wet weight averaged 5.0% in fed snails and 15% in starved snails. 3. 3. Thin layer chromatography (TLC) showed that the main neutral lipid fractions in both fed and starved snails were free sterols and triacylglycerols, along with lesser amounts of sterol esters, free fatty acids and methyl esters. 4. 4. TLC showed a reduction in the free fatty acid and triacylglycerol fractions of starved snails. 5. 5. Oil Red O staining showed the presence of neutral lipids in the liver of fed snails; starved snails were Oil Red O negative.
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More From: Comparative Biochemistry and Physiology -- Part A: Physiology
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