Linkage disequilibrium block single-nucleotide polymorphisms in FTO alpha ketoglutarate dependent dioxygenase gene inference with breast cancer and Type II diabetes in Pakistani female population.

Abstract

Breast Cancer and Diabetes Mellitus are top ranked non-communicable life threatening diseases concerned in South Asia. The growing scientific clues witness the involvement of genetic variations which readily serve as risk factors for the disease onset. Comprehending the association of genetic predictors and risk factors in the conserved genome regions can help reveal underlying disease genetics and identify the sustained druggable targets. The present study aims to identify discrete inference of FTO alpha-ketoglutarate dependent dioxygenase gene linkage disequilibrium block SNPs rs9940128 and rs9939609 as prognostic genetic elements in defining the disease course either as BrC or NIDDM in Pakistani population. Clinically abreast female Breast Cancer and Type II (Non-Insulin Dependent) Diabetes Mellitus cases with the healthy controls participated in the study. The genomic study was established on the DNA of cases and controls through Tetra primers ARMS PCR and PCR-RFLP; data were analyzed statistically to reach comprehensive scientific annotation. Breast Cancer incidence was high in post menopause women. Fretful cholesterol, triglycerides, hypertension, sugar profiles and high incidence in females was evident in Type II (Non-Insulin Dependent) Diabetes Mellitus. BMI and family history were meager factor for either of the diseases. FTO gene alpha-ketoglutarate dependent dioxygenase linkage disequilibrium block Single-Nucleotide Polymorphism rs9939609 and rs9940128 threating inference was significant in the cancer and diabetes subjects correspondingly. The conclusion indicates serious clinical derailments in breast cancer and Type II (Non-Insulin Dependent) Diabetes Mellitus auxiliary to disease complication in genetically risk bearing FTO alpha-ketoglutarate dependent dioxygenase gene haplotype/linkage disequilibrium block SNPs prevailing in the affected Pakistani population. These clinical and genetic indicators could decisively be adopted in health care practice to intervene the sky rising disease incidence.