Abstract
Analysis of 33 randomly selected titrations of herpes simplex on chorioallantoic membranes of embryonated hens' eggs revealed that the variance of pock counts among replicate eggs was considerably higher than expected and that the mean pock counts at high concentrations of virus were less than predicted from counts obtained following infection at low concentration of virus. The curve relating mean pock count to virus concentration leveled off in 26 titrations. The deviation was significant in 4 of the titrations. The upper limit of the linear portion of the dose response curve as defined in the text varied from 11 to 1100 mean pocks per membrane. Methods for estimating virus titers based on linear and parabolic approximations of the pock count-dose relationship were described and compared. The parabolic approximation was recommended for studies in which a more precise estimate of virus titer was desired.
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