孵化鶏卵に馴化せしめたHSTウイルスの免疫学的並びに形態学的研究に就て

Abstract

HST virus is one of filtrable agents successfully isolated from Yoshida tumor in 1950 by Hamazaki passing through the different species of animals, and it has already been verified that immunoserologically it is specific to this tumor and that it is also an indispensable factor for the growth and transplantation of the tumor. This time we carried out immunological and morphological studies on this virus adapted to embryonated eggs.The HST virus successively inoculated into the yolk sack and chorioallantoic membrane of embryonated eggs in the microbiological department of our school can well become adaptation to embryonated eggs, and consequently the chick embryos usually die 4-6 days after the HST virus inoculation. In the liver of chick embryos dissociation, fatty degeneration, and necrobiosis of parenchymal cells, and the infiltration of monocytes and lymphoid cells can patho-anatomically be recognized.It has been found that this virus adapted to the embryonated eggs maintains the properties essentially the same as the original HST virus in that when it is inoculated successively back to its host animals, guinea pigs, inflammatory tissue reaction occur successively, though slightly milder than in the case of original virus.Next, in order to determined whether the virus successfully replaced from the embryonated eggs back to guinea pigs to be the same as the original HST virus, the cross-neutralization of two viruses by antisera were performed and it was proven that the tests were positive. Therefore the former was serologically quite identified with the latter, original HST virus.On the other hand, after purifying the agent in the allantoic fluid and examining it under an electron-microscope, round particles of 30-60mμ in size were obtained.