Light-field Fourier Microscope (FiMic) for transparent objects (Conference Presentation)

Abstract

A new branch of research, dedicated to lightfield, has recently seen an important growth in the microscopist community and it is called integral or lightfield microscopy. One recent implementation of a lightfield microscope is the Fourier integral Microscope (FiMic). In this setup a microlens array (MLA) is placed at the Fourier plane of the objective lens, therefore, the sensor behind each microlens is capturing the spatial information of a different perspective of the sample. The spatio-angular information captured can used to reconstruct the 3D volume. A very wide field of research among microscopists is for objects that have an extremely low contrast or that are completely transparent. In order to obtain a 3D reconstruction of a transparent sample our work has been focused on the combination of the FiMic with a dark-field illumination. In this way a 3D reconstruction of phase objects is achieved.