Abstract
Camelina (Camelina sativa L.) is a potential biofuel crop and beneficial rotation crop in dryland cropping systems. Little is known about camelina microbiota or the legacy effect of soil origin/cropping system zones on camelina-associated microbiome assembly. To explore camelina-microbe associations, we grew camelina in the greenhouse using soil transplanted from 33 locations in the dryland wheat production area of eastern Washington. Bacterial, archaeal and fungal communities from bulk soil, rhizosphere and endosphere were characterized with 16S rRNA and ITS amplicon sequencing and were analyzed alongside site-specific climatic and edaphic data. We found soil from the highest precipitation zone had higher alpha diversity than soil from the driest zone, but this effect was not seen in the greenhouse rhizosphere or endosphere. Plant compartment, cropping system zone, and soil origin all significantly influenced microbial composition with soil pH and organic matter as well as precipitation at origin as major predictors. Analysis of abundance-occupancy distributions suggests that camelina selects for several different Actinobacteria (though distinct genera in the rhizosphere and endosphere). Rhizobium, and Clostridium. Sphingomonas ASVs were also consistently enriched in the rhizosphere, suggesting that they are present in soils collected throughout eastern Washington and may represent good candidate biostimulants. Several lignin decomposing fungi had site-specific rhizospheric distributions suggesting that they may be dispersal-limited or result from the legacy effect of long-term wheat cropping. Overall, this study contributes to our understanding of microbiome assembly in and on camelina roots while also highlighting the potential impact of cropping history on soil and plant-associated microbiomes.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call