Abstract
We measured the constituent triacylglycerol of cotton oil, the only edible oil containing a cyclo fatty acid. Due to the difficulty of obtaining a standard for triacylglycerols containing cyclo fatty acids, cotton oil is analyzed using cyclo triacylglycerol in kapok oil as a reference. Analytical methods used liquid chromatography fast atom bombardment mass spectrometry (LC / FAB-MS) using a matrix of 3% m-NBA containing 3 mM NaCl, and orthogonal transfer method for APCI / TOF-MS. FAB mass spectral properties of triacylglycerols containing cyclo fatty acids can be observed with [M + Na]+, [M-1]+ molecular ions, and [M- (RCOO)] + fragments. In the orthogonal APCI / TOF-MS spectrum, only the [M-1] + molecular ion appears, so that the presence of triacylglycerol containing CPFA becomes apparent. On the other hand, chain triacylglycerol can be observed as [M + 1]+ molecular ions and [M- (RCOO)]+ fragments. Based on these observations, triacylglycerols containing ten types of cyclo fatty acids were successfully successfully detected in crude cottonseed oil. In addition, the APCI/TOF-MS technique successfully detected dilinoleoyl-marvalyl-glycerol and palmtoyl-linoleoyl-marvalyl glycerol in the purified cottonseed oil.
Highlights
IntroductionPrior to the 2000s, numerous studies into cyclo fatty acid-containing triacylglycerols (or cyclopropane fatty acids, CPFAs) were published
Prior to the 2000s, numerous studies into cyclo fatty acid-containing triacylglycerols were published
With improvements in the purification methods available for kapok oil, cyclopropane fatty acid (CPFA)-free kapok oil has become available for purchase in Indonesia, it has yet to be employed in food-based applications
Summary
Prior to the 2000s, numerous studies into cyclo fatty acid-containing triacylglycerols (or cyclopropane fatty acids, CPFAs) were published. Cotton seeds contain approximately 0.16% of cyclo fatty acids, where cottonseed meal contains 0.03% and crude cottonseed oil contains 0.16%. During liquid chromatography (LC) analysis, the cyclo triacylglycerol is saponified and UV-detected as a phenacyl ester derivative [6]. The analysis of cyclo fatty acids is laborious, and a method of directly analyzing cyclo fatty acid-containing triacylglycerols is desired. In this context, Bland et al [9] analyzed the triacylglycerols of cottonseed oil using LC and GC techniques. We analyze the cyclo triacylglycerols present in cottonseed oil using our previously described LC/FAB-MS method
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