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Abstract
SEVERAL years ago studies were initiated in this laboratory to isolate the compounds in crude cottonseed oil which cause pink-colored whites and large salmon-colored yolks in stored eggs produced by hens fed the oil. Since this work was started Masson et al. (1957) reported that sterculic acid from Sterculia foetida seeds caused pink white discoloration, and Faure (1956) showed that sterculic acid gave a positive Halphen reaction. Lorenz (1939) believed the substance in crude cottonseed oil that causes “pink-white” egg discoloration is the same as the one that gives the Halphen reaction or something very closely associated with it. Shenstone et al. (1965) isolated sterculic acid from cottonseed oil by reversed phase chromatography.Shenstone and Vickery (1956) obtained from cottonseed oil a fatty acid fraction that gave a positive Halphen reaction and caused “pink-white” discoloration of stored eggs. Macfarlane et al. (1957) isolated from this fraction a fatty acid, which …
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