Abstract
The Fox Protein Footprinting System is a novel hydroxyl radical protein footprinting (HRPF) method that uses a proprietary flash oxidation lamp to photolyze hydrogen peroxide to form hydroxyl radicals (⋅OH). The ⋅OH irreversibly modify solvent exposed side chains of amino acids. As solvent accessibility changes, the ⋅OH modification concordantly changes. With the addition of real time radical dosimetry, the effective ·OH concentration can be determined and easily adjusted, allowing conditions with varying scavenging effects to be easily compared.
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