Abstract
Background: Adipose-derived stem cells (ASCs) have been developed as a substitute for chondrocytes in cartilage tissue engineering. This study aimed to evaluate the potency of ascorbic acid to induce the chondrogenic differentiation of human ASCs cultured on fibrin substrate. Methods: Human ASCs were isolated by an enzymatic process and characterized. The fibrin scaffold was fabricated from human blood as a natural scaffold. The ASCs were cultured in 10% fetal bovine serum (FBS) supplemented by ascorbic acid in various concentrations (1, 3, 6 mg/ml) and a control without an ascorbic acid supplementation. The chondrogenic differentiation of the ASCs was evaluated involving glycosaminoglycan analysis, and the mRNA expression of type 2 collagen, aggrecan and type 1 collagen. Results: Chondrogenic differentiation occurred when the ASCs were supplemented by 3 mg/ml of ascorbic acid and cultured on a fibrin scaffold confirmed by glycosaminoglycan (GAG) analysis, and the mRNA expression of type 2 collagen and aggrecan. The expression of type 1 collagen, which is an osteogenic marker, was low. Conclusions: ASCs with a supplementation of 3mg/ml of ascorbic acid cultured on a fibrin substrate promotes chondrogenic differentiation.
Highlights
Mesenchymal stem cells (MSCs) are multipotent cells and they have an important role in regenerative medicine therapy 1
This study aimed to evaluate the potency of ascorbic acid to induce the chondrogenic differentiation of human Adipose-derived stem cells (ASCs) cultured on fibrin substrate
Chondrogenic differentiation occurred when the ASCs were supplemented by 3 μg/ml of ascorbic acid and cultured on a fibrin scaffold confirmed by glycosaminoglycan (GAG) analysis, and the mRNA expression of type 2 collagen and aggrecan
Summary
Mesenchymal stem cells (MSCs) are multipotent cells and they have an important role in regenerative medicine therapy 1. The MSCs isolated from adipose tissue are called adipose-derived stem cells (ASCs). The supplementation of vitamin C (ascorbic acid) supports the proliferation of ASCs 9, preventing the cells from aging because the ascorbic is antioxidant 10, alongside induced cell differentiation into chondrogenic or osteogenic 9,11. This study aimed to evaluate the potency of ascorbic acid to induce the chondrogenic differentiation of human ASCs cultured on fibrin substrate. The chondrogenic differentiation of the ASCs was evaluated involving glycosaminoglycan analysis, and the mRNA expression of type 2 collagen, aggrecan and type 1 collagen. Results: Chondrogenic differentiation occurred when the ASCs were supplemented by 3 μg/ml of ascorbic acid and cultured on a fibrin scaffold confirmed by glycosaminoglycan (GAG) analysis, and the mRNA expression of type 2 collagen and aggrecan. Conclusions: ASCs with a supplementation of 3 μg/ml of ascorbic acid cultured on a fibrin substrate promotes chondrogenic differentiation.
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