Lack of in vitro anticancer and antimicrobial activities in Suaeda maritima (seablite) crude extracts

Abstract

Aims: The aim was to determine antimicrobial and anticancer activities for water and ethanol extracts of leaves and shooting parts of seablite. Materials and Methods: Anticancer activity tests of seablite extracts were performed by resazurin microplate assay (REMA) and tested with 3 cell lines including KB cell line, epidermoid carcinoma of the oral cavity (American Type Culture Collection [ATCC] CCL-17), MCF-7 cell line, breast adenocarcinoma (ATCC HTB-22) and NCI-H187 cell line, and small cell lung carcinoma (ATCC CRL-5804). Antimicrobial activities of seablite water and ethanol extracts against herpes simplex virus type-I (HSV-1) and Mycobacterium tuberculosis H 37 Ra strain were tested according by green fluorescent protein-based assay; Candida albicans (ATCC 90028) and Bacillus cereus (ATCC 11778 or TISTR 687) were tested according by REMA; and Plasmodium falciparum , K1 strain was tested according by micro-culture radioisotope techniques. Results: Both extracts were not possessed anticancer activity to KB-oral cavity cancer, MCF7-breast cancer and NCI-H187-small lung cancer cell lines at maximum concentration = 50 μg/mL. In the same way to anticancer activity assays, both extracts were not inhibited HSV-1, P. falciparum K1 strain, M. tuberculosis H 37 Ra strain and B. cereus at the same concentration. Conclusions: Ethanol and water extracts of leaves and shooting parts of seablite had no significant anticancer and antimicrobial activities.