Abstract
Cloves (Syzygium aromaticum) as been used as traditional medicine for many years and they possess antibacterial, antifungal and antiviral properties. Clove is known for its anticancer property on various cancer cell lines and is well established, but its anticancer effect on OSCC cell lines is less known. Aim of the study was to determine the anticancer and antioxidant effect of Syzygium aromaticum extract on OSCC cell lines (KB cell lines) and compare the same with normal mouse fibroblasts cell lines (L292 cell lines). KB cell lines and L292 cell lines were commercially obtained. Clove was obtained from local market and ethanolic extract (EC) of clove was prepared. Anticancer activity was assessed by MTT, neutral red, DAPI and Double staining assay and antioxidant assay was carried out by FRAP, PM and DPPH assay. The antioxidant property of EC of clove increased with increase in the concentration in a dose dependent manner. Both MTT and Neutral Red assay showed increase in cell death with increase in concentration of EC of clove. Double staining and DAPI showed increase in cell death when treated with EC of clove. The anticancer and antioxidant activity of EC of clove was comparable with standard drug used in the assay. This in vitro study demonstrates effective anticancer and antioxidant activity on KB cell lines when compared to standard control. However, further studies are to be conducted in order to characterize other potential antitumor components of the clove, so that it can be used as therapeutic agent in treating oral carcinoma.
Highlights
Cancer is a major public health problem in the world with its statistical numbers continuing to increase [1]
Considering its anticancer activity we did literature search to find out anticancer effect of clove on Oral squamous cell carcinoma (OSCC) cell line and we found lack of studies on effect of Syzygium aromaticum on OSCC cell lines, an in vitro study was designed to determine the effect of Syzygium aromaticum extract on OSCC cell lines (KB cell lines)
OSCC cell lines (KB cell lines) and normal mouse fibroblasts (L292 cell lines) cell lines were procured from NCCS, Pune, which was stored in DMEM with low glucose (Cat No-11965-092)
Summary
Cancer is a major public health problem in the world with its statistical numbers continuing to increase [1]. Oral cancer falls in the top three of all cancers in India and is estimated that over 30% of all cancers reported in the country are oral cancers. The incidence of oral cancer is highest in India, South and South East Asian countries. Therapies for oral cancer are surgical treatment, hormone therapy, radiation therapy, and chemotherapy. These treatment options results with adverse and toxic side effects [2]. The exploration of medicinal plants continues to hold significant promise for the prevention and treatment of cancer with minimal or no side effects. Evidence from World Health Organization states that about 65% of the population across the globe prefer to use traditional or herbal
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