Kriopreservasi untuk Konservasi Plasma Nutfah Tanaman: Peluang Pemanfaatannya di Indonesia

Abstract

<p>Increasing rate of plant germplasm lost in<br />Indonesia has promoted the implementation of various<br />methods for their conservation. Cryopreservation is a technique<br />applicable for a long-term preservation (base collection)<br />of plants possessing non-orthodox (recalcitrant and<br />semi-recalcitrant) seeds and those propagated vegetatively.<br />The technique can be used as an alternative method for<br />orthodox seed plants preservation in the ex situ conservation<br />system. Although field and in vitro collection methods can<br />be applied for the non-orthodox seed plants, a number of<br />disadvantages possesed by these methods, especially in the<br />tropics or the developing countries, deny their use for the<br />establishment of a long-term germplasm collection. Successful<br />implementation of the cryopreservation technique is<br />supported by the development of protocols, which are able<br />to provide a high recovery rate for species understudy, using<br />vitrification based methods which are simple, economical,<br />applicable to complex organs, and able to implement a high<br />number of explants per experiment. The availability of infrastructures<br />including in vitro culture laboratories, continue<br />supply of liquid nitrogen is highly supporting the use of<br />cryopreservation technique in Indonesia.</p>