Abstract
A kinetic-fluorimetric method is proposed for the determination of ascorbic acid. The method is based on the activating effect of ascorbic acid on the aerial oxidation of 2-hydroxynaphthaldehyde thiosemicarbazone in the presence of manganese(II), which acts as a catalyst. The reaction is monitored spectrofluorimetrically by measuring the initial rate at the excitation and emission wavelengths of the oxidation product. A linear calibration graph from 0.15 to 3 µM is obtained. The method has been applied to the determination of ascorbic acid in pharmaceuticals and fruit juices and of ascorbic acid and dehydroascorbic acid in urine.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
