Khảo sát khả năng đối kháng của xạ khuẩn đối với nấm Phytophthora sp. gây bệnh cháy lá, thối thân trên cây sen

Abstract

The research was aimed to screen Actinomycete isolates which are able to control leaf blight and stem rot disease on lotus caused by Phytophthora sp.. Ninety-three actinomycete isolates were collected from lotus field in some provinces of the Mekong Delta. The preliminary testing determined 30 isolates capable inhibiting Phytophthora sp. growth in laboratory conditions. Testing the antagonistic ability against Phytophthora sp. of 30 actinomycete isolates done with 5 replications showed that 5 isolates CM18, HG3, HG4, TG1 and BL6 indicated higher stabler antagonistic ability than others tested and the best - CM18 isolate - could reduce mycelial growth of Phytophthora sp. within the radius of 16.75mm and antagonistic efficacy of 89.89% at 60 hours after inoculation. Besides, the testing β-glucanase productivity of these Actinomycetes on β-glucan medium conducded with 5 replications showed that CM18 isolate was the best with the β-glucan lyses halo radius of 10,81mm at 14 days after testing.Moreover, all tested actinomycete isolates were able to produce siderophore under hydroxamates form.