Abstract

The research was conducted in laboratory and nethouse of Plant Protection Department, Can Tho University to screen actinomycete isolates able to control anthracnose disease on chilli caused by Colletotrichum sp. One hundred actinomycete isolates were collected from chilli field in some provinces of the Mekong Delta. The preliminary testing determined 20 isolates capable to inhibit anthracnose fungus in laboratory conditions. Testing the antagonistic ability against anthracnose fungus of those 20 actinomycete isolates done with 5 replications showed that 3 isolates CT10, VL17 and HG03 could reduce mycelial growth of Colletotrichum sp. within the radius of 13.7mm, 12.3mm and 13.5mm, respectively and the antagonistic efficacy of 49.82%, 44.73% and 49.09%, respectively at 9 days after inoculation. On the other hand, the biocontrol ability of the 3 good actinomycete isolates was tested with 5 replications in nethouse conditions. The results showed that all three actinomycete isolates, CT10, VL17 and HG03 were able to control anthracnose disease on chilli. The treatment with HG03 application duplicated at 2 days before and after inoculation showed as high ability to control the disease as of the Carmanthai 800WP (Carbendazim) treatment as based on narrow lesion diameter (9.12mm) and high efficiency of disease reduce (63.17%) at 9 days after testing.

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