Abstract
Lactic acid bacteria are known to have the ability to produce antimicrobial compounds known as bacteriocins. This study aims to determine the ability of lactic acid bacteria to inhibit the growth of Salmonella thypii bacteria. In this study, the lactic acid bacteria used were a collection of the Biology Laboratory of the University of Medan Area. Eight types of lactic acid bacterial isolates were used, including IN01, IN02, SF01, SF02, NN01, NN02 SPU01 and SPU04. A confirmation test was carried out on the growth of lactic acid bacteria and Salmonella thypii by visual and microcopic confirmation through differential staining. The results showed that all lactic acid bacteria were a group of gram-positive bacteria in the form of bacilli. The survival of lactic acid bacterial isolates in the environment with variations in pH and salt levels was indicated by the number of colonies that grew after the incubation period. The antagonistic ability of lactic acid bacteria in suppressing pathogenic bacteria was proven by the presence of an inhibition zone in the form of a clean area around the pathogenic colony. It is known that the SPU01 and SF01 bacterial isolates have the highest inhibition zone values, namely 29.79 mm and 28.13 mm.
Highlights
PENDAHULUAN Bakteri asam laktat merupakan kelompok bakteri gram positif yang tidak menghasilkan spora, termasuk ke dalam kelompok bakteri gram positif, memiliki kemampuan menghasilkan karbohidrat dan mampu menghasikan asam laktat
This study aims to determine the ability of lactic acid bacteria to inhibit the growth of Salmonella thypii bacteria
The results showed that all lactic acid bacteria were a group of gram-positive bacteria in the form of bacilli
Summary
Rahmiati1)*, Helen Anjelina Simanjuntak2) & Toberni S Situmorang3) 1Universitas Medan Area, Indonesia. Bakteri asam laktat diketahui memiliki kemampuan menghasilkan senyawa antimikroba yang dikenal sebagai bakteriosin. Penelitian ini bertujuan untuk melihat kemampuan bakteri asam laktat dalam menghambat pertumbuhan bakteri Salmonella thypii. Bakteri asam laktat yang digunakan merupakan koleksi Laboratorium Biologi Universitas Medan Area. Delapan jenis isolat bakteri asam laktat yang digunakan antara lain IN01, IN02, SF01, SF02, NN01, NN02 SPU01 dan SPU04. Dilakukan uji konfirmasi terhadap pertumbuhan bakteri asam laktat dan Salmonella thypii dengan melakukan konfirmasi secara visual dan mikrokopis melalui pewarnaan diferensial. Kelangsungan hidup isolat bakteri asam laktat di lingkungan dengan variasi pH dan kadar garam ditunjukkan dengan jumlah koloni yang tumbuh setelah masa inkubasi. Kemampuan antagonis bakteri asam laktat dalam menekan bakteri patogen dibuktikan dengan adanya zona hambat berupa daerah bersih di sekitar koloni patogen. Diketahui bahwa isolat bakteri SPU01 dan SF01 memiliki nilai zona hambat tertinggi yaitu 29,79 mm dan 28,13 mm
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