Karakteristik Kandungan dan Aktivitas Antioksidan Ekstrak Kunyit Putih (Curcuma zedoaria (Berg.) Roscoe)

Abstract

The characteristic of compound and antioxidant activities of kunyit putih (Curcuma zedoaria (Berg.) Roscoe) extracted with alcohol has been studied. The purpose of this study is to determine the characteristic of compound and antioxidant of kunyit putih. The content of total phenol and total flavonoid were determined by visible spectrophotometry method, the activity of antioxidant was measured by the method of Kim and two methods were used to determine reactive oxygen species. The two method were lipid peroxide test with iron thiocyanate and tiobarbitutit acid (TBA). The results showed that the antioxidant activity of kunyit putih includes 1 (one) gram total phenol of crude extract of kunyit putih contains phenol that equivalent to 4.68 mg of galic acid, 1 (one) gram total flavonoid of crude extract of kunyit putih equivalent to 1.83 cuecetin, the capture of free radicals of 4000 µg/mL kunyit putih is 51,76 and it is equal to 10 µg/mL of vitamin C at 51.70. In the test of thiocyanate, the species of reactive oxygen of 50 µg/mL kunyit putih extract in the fifth day was about 1.32 equivalent to 50.µg/mL curcumin at 0.89. In the test of titriburit acid, the species of reactive oxygen of 400 µg/mL of kunyit putih extract in the fifth day was 40.20% fewer than the one from 50 µg/mL curcumin that was 97.64%.