JOINT CULTIVATION OF REPRESENTATIVES OF THE GENERA PROPIONIBACTERIUM, LACTOBACILLUS, BIFIDOBACTERIUM ON UNIFIED MEDIA

Abstract

Probiotic microorganisms are living non-pathogenic, non-toxigenic microorganisms that enter the intestine with food and have a positive effect on the body, normalising the composition and biological activity of the microflora of the digestive tract. Lactic acid bacteria of the genera Lactobacillus and Bifidobacterium occupy a prominent place among the microorganisms that make up probiotics. The presence of these bacteria in the gastrointestinal tract provides an antagonistic environment that is directed against pathogenic microorganisms and creates conditions for the growth and development of beneficial bacteria. Bifidobacterium and Lactobacillus can enhance the absorption capacity of food components, lactose tolerance, stabilise liver function by normalising bowel function and reducing blood ammonia levels. Recently, the spectrum of microorganisms that exhibit antagonistic properties and have a favourable effect on the vital activity of the organism has been expanding. Much attention is paid to propionic acid bacteria, which are part of the microflora of various biotopes. Propionic acid bacteria synthesise vitamin B12, bifidogenic growth factors and bacteriocins, have high adhesive and immunomodulatory properties, and their metabolites have antimutagenic effects. In this study, we analysed the possibility of individual and joint cultivation of representatives of the genera Propionibacterium, Lactobacillus and Bifidobacterium on unified media containing corn and yeast extracts. It was found that these nutrient media are effective for monocultivation of propionic acid bacteria and lactobacilli. At the same time, not only an increase in the optical density of propionic acid bacteria (3-fold), but also an increase in acid formation (2-fold on average) was observed. The possibility of joint cultivation of lactic acid and propionic acid bacteria by creating associations of two types was proved. The number of colonies of the studied microorganisms on selective nutrient media after cocultivation in unified media of both types was determined, the highest survival rate was observed for Propionibacterium. The antagonistic effect of both individual cultures and established associations against certain gram-positive bacteria and microscopic fungi was recorded.