Abstract
Wood, et at.,1 and Tatum, et al.,2 showed that an ether-soluble acid (obtained from yeast-extract, potato-extract or corn-steep) and vitamin B1 stimulate growth of the propionic acid bacteria. Snell, et al.,3 have shown that ether-extract of potato stimulates Lactobacillus delbruckii but an additional factor present in peptone is essential for their growth in hydrolyzed casein, tryptophane and glucose medium.In the present investigation we have continued this study, particularly in determining the effect of lactoflavin on growth. Lava, et al.,4 fractionated yeast-extract and found the portion which contained B2 was the most active. They suggested that flavin or B2 might be a stimulant for the propionic acid bacteria but were unable to prove this because of the impurity of their B2 preparation and lack of a basal medium free of B2.We have tested the activity of lactoflavin by adding it to a basal medium which contained per 100 cc. (1) ether-extract of 3 gm. of yeast-extract, (2) 1.0 gamma B1 (Merck's cryst...
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