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Abstract
Ogura cytoplasmic male sterility (CMS) contributes considerably to hybrid seed production in Brassica crops. To detect the key protein species and pathways involved in Ogura-CMS, we analysed the proteome of the cabbage Ogura-CMS line CMS01-20 and its corresponding maintainer line F01-20 using the isobaric tags for the relative and absolute quantitation (iTRAQ) approach. In total, 162 differential abundance protein species (DAPs) were identified between the two lines, of which 92 were down-accumulated and 70 were up-accumulated in CMS01-20. For energy metabolism in the mitochondrion, eight DAPs involved in oxidative phosphorylation were down-accumulated in CMS01-20, whereas in the tricarboxylic acid (TCA) cycle, five DAPs were up-accumulated, which may compensate for the decreased respiration capacity and may be associated with the elevated O2 consumption rate in Ogura-CMS plants. Other key protein species and pathways involved in pollen wall assembly and programmed cell death (PCD) were also identified as being male-sterility related. Transcriptome profiling revealed 3247 differentially expressed genes between the CMS line and the fertile line. In a conjoint analysis of the proteome and transcriptome data, 30 and 9 protein species/genes showed the same and opposite accumulation patterns, respectively. Nine noteworthy genes involved in sporopollenin synthesis, callose wall degeneration, and oxidative phosphorylation were presumably associated with the processes leading to male sterility, and their expression levels were validated by qRT-PCR analysis. This study will improve our understanding of the protein species involved in pollen development and the molecular mechanisms underlying Ogura-CMS.
Highlights
Cabbage (Brassica oleracea L. var. capitata) is an important leafy vegetable cultivated worldwide, and it provides substantial amounts of fibre, vitamins, mineral elements and health-promoting nutrients
We identified nine differential abundance protein species (DAPs) associated with programmed cell death (PCD), especially, four protein species that are highly related to PCD: the two predicted catalase-3 proteins (Bol006999, and Bol026973, which catalyses the breakdown of hydrogen peroxide into water and oxygen), one allene oxide synthase (Bol035942, which catalyses dehydration of the hydroperoxide to an unstable allene oxide in the JA biosynthetic pathway), and one glycolate oxidase (Bol037061, which encodes a glycolate oxidase that modulates reactive oxygen species-mediated signal transduction)
The present study provided an iTRAQ-based proteome analysis of Ogura-cytoplasmic male sterility (CMS) using the cabbage Ogura-CMS line CMS01-20 and its isogenic maintainer line F01-20
Summary
Cabbage (Brassica oleracea L. var. capitata) is an important leafy vegetable cultivated worldwide, and it provides substantial amounts of fibre, vitamins, mineral elements and health-promoting nutrients. Cross-pollination in hybrid seed production is mainly accomplished using male sterility and self-incompatibility. Self-incompatibility has several limitations, such as poor seed purity and high costs of parental reproduction, whereas male sterility is generally more reliable and economically effective [1,2]. CMS represents the most widely used breeding tool in cabbage hybrid seed production [4,5]. Ogura cytoplasmic male sterility (Ogura-CMS) was discovered in radish [6] and has been transferred to several Brassica species [5,7,8,9]. Ogura-CMS is stable and easy to transfer between species; it has become one of the most important types of CMS in B. oleracea [11]
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