Isolation, Screening and Partial Characterization of Thermophilic Bacteria Producing Protease from Bukik Gadang Hot Springs, Solok Regency

Abstract

Research about the isolation, screening and partial characterization of thermophilic bacteria that produce protease from Bukik Gadang hot springs, Solok Regency, was carried out at the Basic Biology Laboratory, UPT Basic and Central Laboratory, Andalas University in January - April 2022. This study aims to obtain isolates of thermophilic bacteria that indicated protease production and to determine the partial characterization of thermophilic bacteria that indicated protease production from Bukik Gadang hot springs, Solok Regency. This study used a survey method and purposive sampling technique. The results of this study obtained 18 isolates from 20 isolates of thermophilic bacteria which indicated protease production, with one isolate of TPBG-03 having the potential to produce protease. Partial characterization by 10 isolates of thermophilic bacteria indicated protease production was generally Gram positive, bacillus cell shape, spore-forming, catalase positive and motile.Keywords - Hot Springs, Isolation, Protease, Screening, Thermophilic Bacteria