Abstract
Thermophilic bacteria can survive at high temperature, in which hot spring is one of its habitats. Indonesia has many hot springs with potential as a habitat for thermophilic bacteria. The purpose of this study was to obtain isolates thermophilic bacteria from Bora hotspring located in Central Sulawesi. This study applied a descriptive-observational study design, characteristics of bacterial properties identified using conventional methods according to the Bergey’s Manual of Determinative Bacteriology. The study was conducted in 3 stages. The first stage was bacteria cultivation on the appropriate media, followed by stage of isolated and the last stage by identified characteristics of thermophilic bacteria which included microscopic and macroscopic morphology, Physiological and biochemistry test. The results of the isolates indicted 4 representative isolated of thermophilic bacteria from Bora Hot Spring namely TM022, TM023, TM024, TM026. The bacteria isolates obtained were bacillus, coccus and Gram negative and positive, while the physiological test of all isolates were able to grow and showed changes in the medium. This study is useful in providing characteristic of indigenous thermophilic bacteria isolates that produces thermostable enzymes.
Highlights
Indonesia is a country with much geothermal activity and high biodiversity
Thermophilic bacteria are microorganisms that live in extreme condition up to a temperature of 121oC
The tools used during study including microscope, autoclave, oven, incubator, tube rack, laminar air flow, test tube, erlenmeyer, petri dish, hot plate, measuring cup, digital balance, pipette, ose needle and loop, sample bottle, cover glass, and an identification key book “Bergey’s Manual of Determinative Bacteriology “(Madigan et al, 2003) Brock Biology of Microorganism
Summary
Indonesia is a country with much geothermal activity and high biodiversity. Indonesia has the largest geothermal resources in the world, with as many as 252 locations spread over 26 Provinces. The large number of hydrothermal areas, waste composting, petroleum drilling wells and natural gas shows potentials that can be empowered and applied in the development of science. Even studies in the last two decades revealed that 99% of the bacteria present in the environment have not been yet explored for biotechnology applications (Panda et al, 2013; Wijayati et al, 2014). Thermophilic bacteria are microorganisms that live in extreme condition up to a temperature of 121oC. Groups of thermophilic microorganisms produce the most enzymes compared to animals and plants
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