Abstract
Abstract A water-soluble polysaccharide, HM 41 , was obtained from Halenia elliptica D. Don by acidic ethanol fractionation and gel filtration. Its homogeneity was confirmed by chromatography using multiple systems. HM 41 was composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal), glucose (Glc) with a molar ratio of 1.0:5.5:1.8:3.0:9.4:21. The average molecular weight of HM 41 was approximately 1.17 × 10 4 . Periodate oxidation, Smith degradation, methylation and GC, IR, NMR, XRD, GC–MS analysis were used for the structural analysis of HM 41 . Its main chain was composed mainly of β -(1 → 4)Gal, β -(1 → 4)Glc and β -(1 → 6)Glc. β -(1 → 4)Gal were substituted at 6-O and on average there were 14 branches among 23 main chain residues; (1 → 4)Glc had no branch; (1 → 6)Glc were substituted at 3- O and on average there were 9 branches among 14 main chain residues. The side chain was composed of (1 → 3,6)-Rha, (1 → 4)/(1 → 5)-Ara, (1 → 4)/(1 → 5)-Xyl, (1 → 4,6)-Man and (1 → 2)-Glc. The terminal residue was composed of Ara, Xyl, Man, Gal, and Glc. Then, we demonstrated that HM and HM 41 had strong scavenging activities in vitro hydroxyl. Overall, HM and HM 41 may have potential applications in the antioxidants for medical and food industry.
Published Version
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