Abstract
Water-soluble crude polysaccharide(PIP) was extracted from cultured mycelium of the fungus Phellinus igniarius. After ethanol precipitation and sepharose CL-6B gel filtration, the fraction of PIP 1 was obtained, which was shown to be a homogeneous polysaccharide by means of high-performance liquid chromatography. The structure of PIP 1 was determined by using several methods. GC analysis indicates that PIP 1 is composed of the monosaccharides of glucose, galactose, and mannose. Their molar ratio is 3. 70: 4. 06: 1. 00. The molar weight was estimated to be 17 kd via HPLC. IR, GC, partial hydrolysis with acid, periodate oxidation, Smith degradation, methylation, and GC-MS analysis were used for the structural analyses of PIP 1 The results show that PIP 1 has a small quantity of branch structure. The main glycosidic linkage of PIP 1 has a β-configuration. The main chain is made up of a large mass of glucose (1—>3) and few mannose (1—>4); the side chain is composed of glucose (1—>3) and galactose (1—>6); the nonre-duced end is composed of galactose and glucose. The side chains are branched at 6–0 of glucose(1—>3,6) and mannose (1—>4, 6). On an average, there are three branches among 20 residues. It is presumable that the existence of 1, 3-linked Glc in the main and side chains is the main reason for its higher antitumor activity.
Published Version
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