Abstract
Proteases are multipurpose group of enzymes which mainly produced from microorganism and used in various industries such as detergent, silver recovery, food, pharmaceutical, leather and textile industries. This work aimed to isolate protease producing bacteria from local environment for use as silver recovery and reuse of polyester from waste X-ray film. Isolation of protease producer was undertaken using skim milk agar medium. A total of 36 protease positive bacteria were isolated from the study area (Arba Minch fish processing site). Five isolates were screened which had relatively higher clear zone and further examined gelatin removal efficiency of their crude enzymes. One isolate designated as <i>Bacillus sp.</i> THZ14 was selected on the basis of gelatin removal efficiency. The crude enzyme from this strain removed gelatin on waste X-ray film at pH 9, 40°C in 40 min. These properties suggest that protease from <i>Bacillus sp.</i> THZ14 could find potential application in silver recovery process and reuse of polyester sheet which have an economic implication.
Highlights
Almost All form of life on earth constituent’s protease enzyme for their physiological function
Isolation of Proteolytic Bacteria Thirty six protease positive isolates were obtained from the study area
The strains were identified as a protease producer since the formation of clear zone around the colony due to hydrolysis of skimmed milk protein in the medium (Figure 1)
Summary
Almost All form of life (animal, plant and microbes) on earth constituent’s protease enzyme for their physiological function. Microbial proteases are being used as best alternatives to the burning and chemical methods of silver recovery from waste X-ray films. This helps in extracting silver, and the polyester film base can be recycled [7, 8, 9]. Microbial protease has a significant potential for hydrolysis of gelatin layer and as result releases silver from waste photographic film [10, 11, 12] Bearing in mind this critical factor, effort should be directed to search microorganisms which produce proteases with high stability in the environment of silver recovery process. These all contribute toward the idea of clean technology and environmentally friendly approach for the welfare human being
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