Abstract
Proteases are among the most important hydrolytic enzymes that found in every organism to undertake important physiological functions. They are multipurpose enzymes used in various industries such as detergent, silver recovery, food, pharmaceutical, leather, and textile industries. This work aimed to produce protease from indigenous microbes for use as detergent additive. Isolation of protease producer was undertaken using skim milk agar medium. Crude enzyme was characterized in terms of wash and stain removal tests. A total of 188 protease positive bacteria were isolated from seven samples collected from Arba Minch town. Out of 36 alkaline protease producing bacteria, one isolate designated as Bacillus sp. Cab44 was selected. The optimum activity was reached at pH 9 and 50°C. The enzyme was stable in the pH range of 7 to 10. It retained 75%, 86% and 72% activity after one hr pre-incubation at 50°C, in 15% H2O2 and 0.3% commercial detergent respectively. The enzyme activity was increased by Mg2+, Cu2+, and Mn2+, was not affected by Ca2+ but decreased by Zn2+, Hg2+ and Fe2+. It removed stains of blood and egg yolk on cotton cloth at pH 9, 40°C, 5.07 U/ml in 30-40 min. These properties suggest that protease from Bacillus sp. Cab44 could find potential application in detergent industries as good candidate of additive in detergent formulation which have economic implication.
Highlights
Proteases are among the most important hydrolytic group of enzymes that found in every organism to undertake important physiological functions [1]
Animal and plant proteases are of important industrial applications, a large proportion of commercially useful proteolytic enzymes currently available in the market are from microorganisms [2]
Effect of Temperature on Enzyme Activity and Stability The protease produced from Bacillus sp
Summary
Proteases are among the most important hydrolytic group of enzymes that found in every organism (prokaryotes, fungi, plants and animals) to undertake important physiological functions [1]. Animal and plant proteases are of important industrial applications, a large proportion of commercially useful proteolytic enzymes currently available in the market are from microorganisms [2]. Microbial proteases are one of the most important groups of enzymes, used in various industrial processes as food, pharmaceutical and detergent industries, as well as in the preparation of leather, textile and wool, among others [3, 4, 5, 6]. It has promising application in medical usage and management of industrial and household waste. The use of microbial system (enzymes) is the best alternative for generation of pollution free industries
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