Isolation of biofilm formers from aquaculture and study, the effect of antibiofilm activity of chitosan mediated silver nanoparticle

Abstract

Bacterial biofilms are the root cause of biofouling and are due to the agglomeration of microbes and microorganisms on a surface that is surrounded or held together by extracellular polymeric substances. Bacteria produce extracellular polymeric substances (EPS), lipopeptides, and other extracellular matrix proteins, which enable them to adhere on to help them attach to surfaces and bind to one another. These extracellular substances can adhere to metals and varied surfaces, possibly producing toxic compounds such as. sulfhydryl groups, In Aquaculture fam and Ponds such Biofilm forms are of greatest threat by paving the way to settle down for broad diversity of epibiotic organisms. They together can contribute economic impact of Aquaculture and farms and ponds.Chitin, the second most abundant polymer on earth, is a commercially valuable product. The deacetylated form of chitin, chitosan possesses more attractive and useful properties. In this study, the raw material used was the waste of the Spp of shrimp, Penaeus indicus. Chitosan-Ag Nano complex was synthesized and used for its antibiofilm-forming properties. Microbes capable to form biofilm isolated from contaminated sites were isolated and characterized for their identity using microtiter plate assay, tube culture assay, and slide culture assay biofilm formers were observed from two different environments such as paddy fields and ponds. Antibiofilm-forming properties of chitosan nanoparticles were assessed using microtiter plate assay.