Isolation and Screening of Cellulolytic Bacteria From Landfill of Cassava Waste

Abstract

Cassava waste is widely available in West Sumatra in the form of cassava peels and leaves because of the large amount of processed cassava which is the local food of West Sumatra. This cassava waste has the potential to be used as poultry feed, but the main problem is the high crude fiber content in the skin and leaves of cassava. One effective way to reduce crude fiber is by fermentation technology using cellulolytic bacteria as an inoculant. Cellulolytic bacteria isolated from cassava waste disposal soil are more effective in degrading crude fiber in cassava waste. The soil sample used for bacterial isolation came from cassava waste disposal soil taken at a depth of 10 cm. Isolation of cellulolytic bacteria was carried out on agar media containing carboxymethyl cellulose (CMC) and screening was carried out using 1% congo red with indications of the formation of clear zones at area coloni and Enzyme activity test using spectrophotometry. The results of the isolation were 5 isolates of cellulolytic bacteria which were characterized by the formation of clear zones around the colonies with the respective cellulolytic indexs (T17 = 1.63), (T16 = 2.05), (T20 = 1.75), (T22 = 1.85) and (T11 = 0.81). The five isolates were gram-positive bacteria and based on the microscopic test the five isolates were bacil bacteria and there are 2 isolates that have high cellulolytic degradation values and enzyme activity, namely T16 and T22