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Abstract
RNA polymerase (nucleoside triphosphate:RNA nucleotidyltransferase, DNA dependent, EC 2.7.7.6) was bound to T4 DNA in the presence of poly(inosinic acid) (poly(I)). It was shown that poly(inosinic acid) can compete for unspecifically bound polymerase but does not seem to influence a certain portion of RNA polymerase bound to, or close to, the promoter region. The DNA sections to which RNA polymerase was bound in the presence of poly(inosinic acid) were isolated and partially characterized: they are double-stranded DNA fragments of uniform size, with a chain length shorter than that of tRNA Phe which is composed of 75 nucleotides. These DNA fragments can serve as templates for transcription by RNA polymerase. From the preferential incorporation of A and U into the transcription product it was concluded that the DNA fragments are rich in A-T. By two-dimensional thin-layer chromatography of their oligopyrimidines it was shown that they have a base composition different from that of average T4 DNA and therefore cannot be random DNA pieces.
Published Version
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