Abstract
SPECIFIC sequences of nucleic acids such as ribosome binding sites1 (W. Gilbert, cited in ref. 2) and portions of the promoter region3,4 have been isolated by protecting those sequences with the relevant protein or organelle and digesting away the exposed nucleic acid. We have isolated specific sequences from the promoter regions in bacteriophage ϕX174 replicative form (RF) DNA using RNA polymerase as the protecting protein. We wish to describe that isolation and the procedures used to localize these RNA-polymerase-protected sequences within the ϕX174 genome.
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