Abstract
To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next‐generation sequencing. Three hundred and sixty‐five (365) individuals were analyzed within fifteen (15) West African populations. The number of alleles for these loci varied from 4 to 30, and the heterozygosity varied from 0.23 to 0.82. The seventeen (17) primers designed here will allow characterizing the genetic diversity of this threaten species on its natural stands and to better understand the population differentiation mechanisms shaping it.
Highlights
The present study describes a new simple sequence repeats (SSR) set designed for P. erinaceus and its use to describe the genetic diversity of three hundred and sixty-five (365) individual trees originating from West Africa
On the basis of descriptors related to leaves, fruits, and seeds, a morphotype adapted to the dry Sudanian zone was described in Oti-Kéran and Fazao-Malfakassa sites, a morphotype adapted to the semiwet Sudano-Guinean zone was described in Abdoulaye site, and a third morphotype adapted to the wet Guinean zone was described in Akposso and Togodo sites
Thirty (30) microsatellites primers were developed based on P. erinaceus populations from three different African countries by using next-generation sequencing (NGS) (Illumina MiSeq sequencing technology)
Summary
Pterocarpus erinaceus Poir. (Lamarck & Poiret, 1823) is commonly known as African rosewood. The present study describes a new SSR set designed for P. erinaceus and its use to describe the genetic diversity of three hundred and sixty-five (365) individual trees originating from West Africa. Our genomic library was constructed using DNA of P. erinaceus samples from twelve randomly selected individuals among populations (Table 1). Cross-amplification tests using the SSR developed by Muller et al (2006) on P. officinalis were performed using P. erinaceus individuals from different sampling sites except for Tamou reserve (Niger). Ligation reaction was performed in a PCR tube containing 45μl of fragmented DNA to which was added successively 20 μl of ligation buffer (5X), 10μl of DNA ligase, 10 μl of water, and 2.5μl of DNA illumina adapter. A 500 cycles NANO V2 cartridge Illumina (2 x 250 pb) was used to sequence the library
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