Isolation and Characterization of Inulinase Producing Bacteria Dahlia hybrida Hort

Abstract

The use of enzymes in the industrial world is growing, one of which is inulinase. This enzyme can hydrolyze inulin to fructose. The use of fructose as a food or beverage sweetener is more beneficial than sucrose. Inulinase can be produced by plants containing inulin and microorganisms. The ability of bacteria to produce inulinase is more effective and efficient because it is able to live in an extreme environment and is easily manipulated genetically. The purpose of this study was to isolate and determine the characteristics of inulinase-producing bacteria from the Dahlia hybrida Hort rhizosphere. This research was conducted using a descriptive method which was carried out in the Laboratory of Microbiology, Biotechnology, and Biochemistry, Faculty of Mathematics and Natural Sciences, UNP and the Biotechnology Laboratory of Andalas University. The working procedure is inulin extraction, bacterial isolation, morphological, microscopic and molecular observations which include the process of bacterial DNA isolation and amplification using the 16S rRNA gene. Data on bacterial characteristics were analyzed descriptively including morphology, microscopy, and molecular.The results obtained were four isolates of inulinase-producing bacteria which were classified as gram-negative bacteria with bacillary-shaped cells. However, it has a different colony morphology, namely LK1 isolate irregular shape and uneven colony edges, LK2 isolate round and flat colony edges, LK3 isolate in the form of roots and branched colony edges like fine threads and LK4 isolate round and flat colony edges. DNA isolation of inulinase-producing bacteria was successfully carried out, but amplification of the 16S rRNA gene was not successful. This is probably due to the not optimal annealing temperature in the PCR cycle.