Abstract
Cell-wall fractions have been prepared from an alcohol-insoluble-residue of carrot root by treatment with ( a) Pronase to remove the cytoplasmic proteins, ( b) hot dilute acid and cold dilute alkali to give pectin-free residues, and ( c) concentrated alkali to leave the α-cellulose and lignin. The purified cell-wall material still contained ∼ 1% protein and was composed mainly of cellulose, lignin, methyl-esterified galacturonic acid, and smaller amounts of galactose and arabinose. Methylation analysis of the insoluble residues indicated the presence, in order of decreasing concentration, of rhamnogalacturonan with the rhamnosyl residues carrying side chains at position 4, cellulose, (1→4)-linked galactan, (1→5)-linked arabinan, (1→4)-linked xylan, (1→4)-linked mannan, and xyloglucan.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call