Abstract
AbstractA method of measuring in vivo nitric oxide (NO) levels is required to detect pathological conditions in which endogenous production is decreased or to identify agents able to release this biomolecule. Unfortunately, nitric oxide has a very short biological half-life and is very difficult to measure. Assay of the oxidative products’ of NO levels, nitrite (NORabbits were used as experimental animals, a validated HPLC-UV/VIS method was used for speciation of nitrite and nitrate. The following substances were administered: blank; “negative blank”: phenyl-N-tert-butylnitrone (PBN); “positive blank” (nitroglycerin); nitrite.PBN administration significantly increased nitrate and decreased nitrite levels, nitrite administration excessively increased nitrate levels, while nitroglycerin (1 mg/kg) significantly increased both nitrate and nitrite levels.Results show that NOx test cannot be considered accurate in acute nitric oxide status testing. Nitrite alone should be used as an in vivo released nitric oxide marker.
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